--- title: Hyperinsulinemic euglycemic clamp at Profil description: "Hyperinsulinemic euglycemic clamp is the gold standard to study insulin action. Profil is the leading institute for glucose clamps with our own device: ClampArt" --- [![profil-logo-wortbild-with claim-4c-offset_1000px](https://www.profil.com/hs-fs/hubfs/profil-logo-wortbild-with%20claim-4c-offset_1000px.png?width=250&name=profil-logo-wortbild-with%20claim-4c-offset_1000px.png "profil-logo-wortbild-with claim-4c-offset_1000px")](https://www.profil.com?hsLang=en-us) [![Contact](https://no-cache.hubspot.com/cta/default/481167/1f89c7d1-107f-4c8d-aa7b-f85d0dce14da.png)](https://cta-redirect.hubspot.com/cta/redirect/481167/1f89c7d1-107f-4c8d-aa7b-f85d0dce14da) [Patient information](https://www.profil.de) # [![Header_Pic1.png](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/Header/Header_1077x260px_Clamp_Art_3.jpg?width=1077&height=260&name=Header_1077x260px_Clamp_Art_3.jpg) Glucose Clamp Discover how Profil is innovating the world of the hyperinsulinemic euglycemic clamp with our proprietary ClampArt® device.](https://www.profil.com/services/hyperinsulinemic-euglycemic-clamp#) ## Automated glucose clamp techniques Profil is the largest glucose clamp study center in the world. We have the capabilities to run up to 28 continuous glucose clamps in parallel giving 24-hour coverage 7 days a week. With several thousands of clamp hours per year, we have extensive experience with all the various clamp designs, including the most commonly used hyperinsulinemic euglycemic clamp. ### The principles of the glucose clamp procedure Glucose clamp experiments are characterized by the measurement of the glucose-lowering effect of a compound by means of a variable glucose infusion rate (GIR), so that blood glucose concentrations are maintained or “clamped” at a predefined target level. GIR is used as a surrogate marker for the [pharmacodynamic effect of a glucose-lowering drug](https://www.profil.com/services/pharmacodynamics?hsLang=en-us), such as exogenous insulin. A glucose clamp study can be performed manually or with automated glucose clamps. Both techniques require considerable experience to provide reproducible results. The advantage of the automated clamp technique is that it provides continuous glucose monitoring and the possibility to adapt GIR on a minute-by-minute basis, whereas in manual clamps the GIR adjustments are performed less frequently (at 3- to 10-minute intervals). ### Developing ClampArt® In 1979, De Fronzo et al. published the first description of a glucose clamp procedure . The founders of Profil, Tim Heise and Lutz Heinemann have worked for many years on refining the operation of the automated glucose clamp technique, seeking ways to improve the performance of the devices and make the experience easier for clinical trial subjects. Having lengthy experience with the Biostator, which was developed in the early 1970s by Life Science Instruments, a project team at Profil headed by [Carsten Benesch](https://info.profil.com/contact-carsten-benesch?hsLang=en-us) developed ClampArt®, a modern glucose clamp device that meets all EU regulatory standards. Its CE-certification was awarded in 2013. The state-of-the-art technology uses of modern sensors and infusion pumps to provide improved quality and utility. Confounding factors like disturbances in blood flow are taken into account and automatically corrected based on continuous determination of blood glucose and the blood dilution factor. ClampArt® provides more reliable blood glucose results than the Biostator and has more refined [GIR calculation algorithms](https://blog.profil.com/blog/improved-algorithm-for-automated-glucose-clamps?hsLang=en-us), meaning better reduction of blood glucose and GIR fluctuations. Profil is renowned for expertise and competence in the assessment of the glucodynamic activity of all kinds of insulin formulations. The exact clamp design is carefully adapted to the study objectives and the compound studied, even if this means using ultra-long clamps with a duration of 52 hours. Due to the unrivalled clamp quality achieved with ClampArt® the pharmacodynamic effects of blood glucose-lowering agents can be determined with the high precision and accuracy demanded by EU regulatory authorities, in particular for biosimilar insulins. Other study objectives require different clamp designs, such as: - The one- or two-step hyperinsulinemic euglycemic clamp, which is considered the gold standard for the assessment of insulin sensitivity - The hyperglycemic clamp for the determination of [beta-cell function](https://blog.profil.com/blog/molecular-pathways-of-diabetes-beta-cell-dysfunction-and-insulin-signalling-in-microvascular-disorders?hsLang=en-us) - The hypoglycemic clamp for the quantification of counter-regulation  - The [pancreatic clamp](https://www.profil.com/services/pancreatic-clamp?hsLang=en-us), used to determine the metabolic role of alpha- and beta-cell hormones, among other things. Read more about **2-step insulin sensitivity clamp** in our factsheet [here](https://www.profil.com/hubfs/2-step%20insulin%20sensitivity%20clamp_02.12.2021_final.pdf?hsLang=en-us).  [Metabolic fluxes](https://www.profil.com/services/stable-isotopes?hsLang=en-us) can also be assessed when combining glucose clamps with the isotope dilution technique using, for example, [6.6-2H2]-glucose to assess endogenous glucose production, or [1.1.2.3.3]-2H5-glycerol to determine lipolysis. ### Other glucose clamp techniques at Profil In addition to these clamp techniques, our expert teams can perform evaluations of oxidative and non-oxidative glucose disposal (using indirect calorimetry); fat oxidation assessments using [1-13C]-oleate and other methods; assessment of adipose tissue lipolysis via microdialysis; and vascular function monitoring during glucose clamp procedures using flow-mediated dilation (FMD), tonometry and/or venous occlusion plethysmography.     **Learn more about [Glucose Clamps](https://info.profil.com/webinar-on-demand-glucose-clamps?hsLang=en-us) or read about [Pharmacodynamics clinical studies](https://www.profil.com/services/pharmacodynamics?hsLang=en-us) at Profil. ** ![pikto2.png](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/piktos/Icon_NewWebinar.png?width=62&height=70&name=Icon_NewWebinar.png) ### Online Seminar   On-Demand Online Seminar: **"Understanding the results** **of glucose clamp studies"**           [![Watch now](https://no-cache.hubspot.com/cta/default/481167/53f3eed6-bc1b-4f4d-9928-2368f1efc934.png)](https://cta-redirect.hubspot.com/cta/redirect/481167/53f3eed6-bc1b-4f4d-9928-2368f1efc934) ![Dr. Carsten Benesch](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/content-bilder/Glucose_Clamp/Glucose_Clamp_Benesch_Mitarbeiter.png?width=154&height=176&name=Glucose_Clamp_Benesch_Mitarbeiter.png) ### Our expert **Dr. Carsten Benesch** Director Technical Development [![Contact](https://no-cache.hubspot.com/cta/default/481167/2a469456-4b63-473e-b149-ff8431ebf786.png)](https://cta-redirect.hubspot.com/cta/redirect/481167/2a469456-4b63-473e-b149-ff8431ebf786) ![Download_grau](https://www.profil.com/hs-fs/hubfs/Download_grau.png?width=70&height=70&name=Download_grau.png) ### Download Methodology fact sheet: **Hypoglycemic glucose clamp studies**     [![Download](https://no-cache.hubspot.com/cta/default/481167/95ea3ec2-aaaf-4100-84b2-48d4cd668f8d.png)](https://cta-redirect.hubspot.com/cta/redirect/481167/95ea3ec2-aaaf-4100-84b2-48d4cd668f8d) ![Download_grau](https://www.profil.com/hs-fs/hubfs/Download_grau.png?width=70&height=70&name=Download_grau.png) ### Download Methodology fact sheet: **2-step insulin sensitivity clamp** [![Download](https://no-cache.hubspot.com/cta/default/481167/9d5bffdd-fd3a-43be-b61a-e590a801c31b.png)](https://cta-redirect.hubspot.com/cta/redirect/481167/9d5bffdd-fd3a-43be-b61a-e590a801c31b) ![Download_grau](https://www.profil.com/hs-fs/hubfs/Download_grau.png?width=70&height=70&name=Download_grau.png) ### Download Methodology fact sheet: **Automated euglycemic glucose clamps**   [![Download](https://no-cache.hubspot.com/cta/default/481167/2d653cf3-0d02-42b5-865e-9765c3397af2.png)](https://cta-redirect.hubspot.com/cta/redirect/481167/2d653cf3-0d02-42b5-865e-9765c3397af2) ![Pikto_Testimonials](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/piktos/Pikto_Testimonials.png?width=81&height=90&name=Pikto_Testimonials.png) ### "The ClampArt® device is special. We were looking at many different sites, where we would do clamp testing and none of those sites had anything similar to the ClampArt® device."  **Dr. Sabih Chaudhry, CEO, Afon Technology Ltd.** [![Content_Pic2.png](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/content-bilder/Glucose_Clamp/Glucose_Clamp_Content1_220.png?width=220&height=220&name=Glucose_Clamp_Content1_220.png) ![enlarge](https://www.profil.com/hubfs/BLUE_MOON/piktos/Lupe-01-1.svg) ](https://www.profil.com/hubfs/BLUE_MOON/content-bilder/Glucose_Clamp/Improved_clamp_algorithm.jpg?hsLang=en-us) ![Content_Pic2.png](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/content-bilder/Glucose_Clamp/ClampArt_1.png?width=220&height=345&name=ClampArt_1.png) ![blank.jpg](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/piktos/Icon_References.png?width=49&height=49&name=Icon_References.png) #### References We have published with the following sponsors: ![Referenzen](https://www.profil.com/hubfs/BLUE_MOON/content-bilder/Referenzen.gif "Referenzen") [View more](https://www.profil.com/knowledge-center/references?hsLang=en-us) ![blank.jpg](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/piktos/Icon_Certificates.png?width=44&height=49&name=Icon_Certificates.png) #### Certificates | ![13485_Zertifikatssiegel_black_A3](https://www.profil.com/hubfs/BLUE_MOON/piktos/ISO_134852016_certificate_blue.png) ISO 13485:2016 ![27001_Zertifikatssiegel](https://www.profil.com/hubfs/BLUE_MOON/piktos/ISO_27001_certificate_.png) ISO 27001 | ![9001:2015_Zertifikatssiegel](https://www.profil.com/hubfs/BLUE_MOON/piktos/ISO_9001-2015_certificate.png) ISO 9001:2015 ![9001:2015_Zertifikatssiegel](https://www.profil.com/hubfs/BLUE_MOON/piktos/BVMA_Logo_Certificate_size.png) BVMA Member | | --- | --- | ![blank.jpg](https://www.profil.com/hs-fs/hubfs/BLUE_MOON/piktos/Icon_Publications.png?width=44&height=49&name=Icon_Publications.png) #### Publications Benesch, C. et al. 2021. New Clamp-PID Algorithm for Automated Glucose Clamps Improves Clamp Quality. J Diabetes Sci Technol. 2021 Feb 10;1932296821991514. [View all](https://www.profil.com/knowledge-center/publications?hsLang=en-us) Continuous glucose monitoring research is one of many important techniques offered by the Profil Institute for Metabolic Research in diabetes-focused clinical investigations. Our glucose clamp system, ClampArt, provides continuous glucose monitoring and we have the capabilities to run up to 26 continuous glucose clamps in parallel giving 24-hour coverage 7 days a week. ###### Profil Institut für Stoffwechselforschung GmbH Hellersbergstraße 9 · 41460 - Neuss, Germany Telefon +49-2131-4018-345 · marketing@profil.com [Imprint](https://www.profil.com/imprint?hsLang=en-us) | [Privacy Policy](https://www.profil.com/privacy?hsLang=en-us)